Construct: shRNA TRCN0000424870

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000443.3-455s21c1
DNA Barcode:
GTTCTTGTTGCTGCCTATATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ABCB4 (5244)
Gene Description:
ATP binding cassette subfamily B member 4
Transcript:
RefSeq NM_000443.3 (NON-CURRENT)
Match location:
Position 455 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000424870)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTTCTTGTTGCTGCCTATATA
Hairpin Sequence:
5'-CCGG-GTTCTTGTTGCTGCCTATATA-CTCGAG-TATATAGGCAGCAACAAGAAC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTTCTTGTTGCTGCCTATATACTCGAGTATATAGGCAGCAACAAGAACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTTCTTGTTGCTGCCTATATACTCGAGTATATAGGCAGCAACAAGAAC-3'

Other clones with same target sequence:

(none)