Construct: shRNA TRCN0000424948

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017763.4-3318s21c1
DNA Barcode:
AGTGTGCTCCAGATGTGTTTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RNF43 (54894)
Gene Description:
ring finger protein 43
Transcript:
RefSeq NM_017763.4 (NON-CURRENT)
Match location:
Position 3318 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000424948)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGTGTGCTCCAGATGTGTTTG
Hairpin Sequence:
5'-CCGG-AGTGTGCTCCAGATGTGTTTG-CTCGAG-CAAACACATCTGGAGCACACT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGTGTGCTCCAGATGTGTTTGCTCGAGCAAACACATCTGGAGCACACTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGTGTGCTCCAGATGTGTTTGCTCGAGCAAACACATCTGGAGCACACT-3'

Other clones with same target sequence:

(none)