Construct: shRNA TRCN0000424976

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001112.3-1656s21c1
DNA Barcode:
TTATACACAACTTGAGCTTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ADARB1 (104)
Gene Description:
adenosine deaminase RNA specific B1
Transcript:
RefSeq NM_001112.3 (NON-CURRENT)
Match location:
Position 1656 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000424976)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTATACACAACTTGAGCTTTA
Hairpin Sequence:
5'-CCGG-TTATACACAACTTGAGCTTTA-CTCGAG-TAAAGCTCAAGTTGTGTATAA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTATACACAACTTGAGCTTTACTCGAGTAAAGCTCAAGTTGTGTATAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTATACACAACTTGAGCTTTACTCGAGTAAAGCTCAAGTTGTGTATAA-3'

Other clones with same target sequence:

(none)