Construct: shRNA TRCN0000425013

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144976.3-2089s21c1
DNA Barcode:
GAATTGAGTCTCATCCATATC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZNF564 (163050)
Gene Description:
zinc finger protein 564
Transcript:
RefSeq NM_144976.3 (NON-CURRENT)
Match location:
Position 2089 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000425013)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAATTGAGTCTCATCCATATC
Hairpin Sequence:
5'-CCGG-GAATTGAGTCTCATCCATATC-CTCGAG-GATATGGATGAGACTCAATTC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAATTGAGTCTCATCCATATCCTCGAGGATATGGATGAGACTCAATTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAATTGAGTCTCATCCATATCCTCGAGGATATGGATGAGACTCAATTC-3'

Other clones with same target sequence:

(none)