Construct: shRNA TRCN0000425019

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020463.2-2581s21c1
DNA Barcode:
TTGGTTGGCTTAGTGGATTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PPP4R3B (57223)
Gene Description:
protein phosphatase 4 regulatory subunit 3B
Transcript:
RefSeq NM_020463.2 (NON-CURRENT)
Match location:
Position 2581 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000425019)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTGGTTGGCTTAGTGGATTAT
Hairpin Sequence:
5'-CCGG-TTGGTTGGCTTAGTGGATTAT-CTCGAG-ATAATCCACTAAGCCAACCAA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTGGTTGGCTTAGTGGATTATCTCGAGATAATCCACTAAGCCAACCAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTGGTTGGCTTAGTGGATTATCTCGAGATAATCCACTAAGCCAACCAA-3'

Other clones with same target sequence:

(none)