Construct: shRNA TRCN0000425028

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175114.3-1348s21c1
DNA Barcode:
AGACGGTGAGGAACGTCAATC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Trak1 (67095)
Gene Description:
trafficking protein, kinesin binding 1
Transcript:
RefSeq NM_175114.3 (CURRENT)
Match location:
Position 1348 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000425028)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGACGGTGAGGAACGTCAATC
Hairpin Sequence:
5'-CCGG-AGACGGTGAGGAACGTCAATC-CTCGAG-GATTGACGTTCCTCACCGTCT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGACGGTGAGGAACGTCAATCCTCGAGGATTGACGTTCCTCACCGTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGACGGTGAGGAACGTCAATCCTCGAGGATTGACGTTCCTCACCGTCT-3'

Other clones with same target sequence:

(none)