Construct: shRNA TRCN0000425086

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015646.4-893s21c1
DNA Barcode:
GGCGGATGAAAGCTACTATAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RAP1B (5908)
Gene Description:
RAP1B, member of RAS oncogene family
Transcript:
RefSeq NM_015646.4 (NON-CURRENT)
Match location:
Position 893 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000425086)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGCGGATGAAAGCTACTATAT
Hairpin Sequence:
5'-CCGG-GGCGGATGAAAGCTACTATAT-CTCGAG-ATATAGTAGCTTTCATCCGCC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGCGGATGAAAGCTACTATATCTCGAGATATAGTAGCTTTCATCCGCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGCGGATGAAAGCTACTATATCTCGAGATATAGTAGCTTTCATCCGCC-3'

Other clones with same target sequence:

(none)