Construct: shRNA TRCN0000425138

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_152672.5-1296s21c1
DNA Barcode:
TTGACCAAATGGGAAGCATTC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SLC51A (200931)
Gene Description:
solute carrier family 51 alpha subunit
Transcript:
RefSeq NM_152672.5 (NON-CURRENT)
Match location:
Position 1296 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000425138)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTGACCAAATGGGAAGCATTC
Hairpin Sequence:
5'-CCGG-TTGACCAAATGGGAAGCATTC-CTCGAG-GAATGCTTCCCATTTGGTCAA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTGACCAAATGGGAAGCATTCCTCGAGGAATGCTTCCCATTTGGTCAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTGACCAAATGGGAAGCATTCCTCGAGGAATGCTTCCCATTTGGTCAA-3'

Other clones with same target sequence:

(none)