Construct: shRNA TRCN0000425240

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005247.2-940s21c1
DNA Barcode:
AGAGACTGTGGTACGTGTCTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FGF3 (2248)
Gene Description:
fibroblast growth factor 3
Transcript:
RefSeq NM_005247.2 (NON-CURRENT)
Match location:
Position 940 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000425240)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGAGACTGTGGTACGTGTCTG
Hairpin Sequence:
5'-CCGG-AGAGACTGTGGTACGTGTCTG-CTCGAG-CAGACACGTACCACAGTCTCT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGAGACTGTGGTACGTGTCTGCTCGAGCAGACACGTACCACAGTCTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGAGACTGTGGTACGTGTCTGCTCGAGCAGACACGTACCACAGTCTCT-3'

Other clones with same target sequence:

(none)