Construct: shRNA TRCN0000425285

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_152551.3-583s21c1
DNA Barcode:
AGCGCTCTGATTCTCAAATTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SNRNP48 (154007)
Gene Description:
small nuclear ribonucleoprotein U11/U12 subunit 48
Transcript:
RefSeq NM_152551.3 (NON-CURRENT)
Match location:
Position 583 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000425285)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGCGCTCTGATTCTCAAATTA
Hairpin Sequence:
5'-CCGG-AGCGCTCTGATTCTCAAATTA-CTCGAG-TAATTTGAGAATCAGAGCGCT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGCGCTCTGATTCTCAAATTACTCGAGTAATTTGAGAATCAGAGCGCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGCGCTCTGATTCTCAAATTACTCGAGTAATTTGAGAATCAGAGCGCT-3'

Other clones with same target sequence:

(none)