Construct: shRNA TRCN0000425293

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145222.2-420s21c1
DNA Barcode:
CAATTCCTTCAGGATCCTTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
B3gnt7 (227327)
Gene Description:
UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 7
Transcript:
RefSeq NM_145222.2 (CURRENT)
Match location:
Position 420 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000425293)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAATTCCTTCAGGATCCTTTA
Hairpin Sequence:
5'-CCGG-CAATTCCTTCAGGATCCTTTA-CTCGAG-TAAAGGATCCTGAAGGAATTG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAATTCCTTCAGGATCCTTTACTCGAGTAAAGGATCCTGAAGGAATTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAATTCCTTCAGGATCCTTTACTCGAGTAAAGGATCCTGAAGGAATTG-3'

Other clones with same target sequence:

(none)