Construct: shRNA TRCN0000425423

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001372.3-3426s21c1
DNA Barcode:
GCCAACCTGGATGCGTTTATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DNAH9 (1770)
Gene Description:
dynein axonemal heavy chain 9
Transcript:
RefSeq NM_001372.3 (NON-CURRENT)
Match location:
Position 3426 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000425423)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAACCTGGATGCGTTTATA
Hairpin Sequence:
5'-CCGG-GCCAACCTGGATGCGTTTATA-CTCGAG-TATAAACGCATCCAGGTTGGC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAACCTGGATGCGTTTATACTCGAGTATAAACGCATCCAGGTTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAACCTGGATGCGTTTATACTCGAGTATAAACGCATCCAGGTTGGC-3'

Other clones with same target sequence:

(none)