Construct: shRNA TRCN0000425454

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015939.3-1649s21c1
DNA Barcode:
AGGCATTATACAGTTAGTAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TRMT6 (51605)
Gene Description:
tRNA methyltransferase 6
Transcript:
RefSeq NM_015939.3 (NON-CURRENT)
Match location:
Position 1649 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000425454)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGGCATTATACAGTTAGTAAT
Hairpin Sequence:
5'-CCGG-AGGCATTATACAGTTAGTAAT-CTCGAG-ATTACTAACTGTATAATGCCT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGGCATTATACAGTTAGTAATCTCGAGATTACTAACTGTATAATGCCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGGCATTATACAGTTAGTAATCTCGAGATTACTAACTGTATAATGCCT-3'

Other clones with same target sequence:

(none)