Construct: shRNA TRCN0000425462

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016233.2-2426s21c1
DNA Barcode:
TGATTCTGCTTTGGTCCAATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PADI3 (51702)
Gene Description:
peptidyl arginine deiminase 3
Transcript:
RefSeq NM_016233.2 (CURRENT)
Match location:
Position 2426 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000425462)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGATTCTGCTTTGGTCCAATT
Hairpin Sequence:
5'-CCGG-TGATTCTGCTTTGGTCCAATT-CTCGAG-AATTGGACCAAAGCAGAATCA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGATTCTGCTTTGGTCCAATTCTCGAGAATTGGACCAAAGCAGAATCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGATTCTGCTTTGGTCCAATTCTCGAGAATTGGACCAAAGCAGAATCA-3'

Other clones with same target sequence:

(none)