Construct: shRNA TRCN0000425491

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016894.3-993s21c1
DNA Barcode:
GATGCACAGTTTGTGATTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ramp1 (51801)
Gene Description:
receptor (calcitonin) activity modifying protein 1
Transcript:
RefSeq NM_016894.3 (CURRENT)
Match location:
Position 993 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000425491)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATGCACAGTTTGTGATTAAA
Hairpin Sequence:
5'-CCGG-GATGCACAGTTTGTGATTAAA-CTCGAG-TTTAATCACAAACTGTGCATC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATGCACAGTTTGTGATTAAACTCGAGTTTAATCACAAACTGTGCATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATGCACAGTTTGTGATTAAACTCGAGTTTAATCACAAACTGTGCATC-3'

Other clones with same target sequence:

(none)