Construct: shRNA TRCN0000425544

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024688.2-1326s21c1
DNA Barcode:
GCTTTGGGAAGACGCTTATTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CCDC7 (79741)
Gene Description:
coiled-coil domain containing 7
Transcript:
RefSeq NM_024688.2 (NON-CURRENT)
Match location:
Position 1326 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000425544)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTTGGGAAGACGCTTATTA
Hairpin Sequence:
5'-CCGG-GCTTTGGGAAGACGCTTATTA-CTCGAG-TAATAAGCGTCTTCCCAAAGC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTTGGGAAGACGCTTATTACTCGAGTAATAAGCGTCTTCCCAAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTTGGGAAGACGCTTATTACTCGAGTAATAAGCGTCTTCCCAAAGC-3'

Other clones with same target sequence:

(none)