Construct: shRNA TRCN0000425554

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018227.5-812s21c1
DNA Barcode:
ACAGGTTTAAATGGATCTATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
UBA6 (55236)
Gene Description:
ubiquitin like modifier activating enzyme 6
Transcript:
RefSeq NM_018227.5 (NON-CURRENT)
Match location:
Position 812 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000425554)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACAGGTTTAAATGGATCTATA
Hairpin Sequence:
5'-CCGG-ACAGGTTTAAATGGATCTATA-CTCGAG-TATAGATCCATTTAAACCTGT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACAGGTTTAAATGGATCTATACTCGAGTATAGATCCATTTAAACCTGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACAGGTTTAAATGGATCTATACTCGAGTATAGATCCATTTAAACCTGT-3'

Other clones with same target sequence:

(none)