Construct: shRNA TRCN0000425576

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013304.2-846s21c1
DNA Barcode:
ACAGTGTTGCATCCGCTTTAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZDHHC1 (29800)
Gene Description:
zinc finger DHHC-type containing 1
Transcript:
RefSeq NM_013304.2 (NON-CURRENT)
Match location:
Position 846 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000425576)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACAGTGTTGCATCCGCTTTAC
Hairpin Sequence:
5'-CCGG-ACAGTGTTGCATCCGCTTTAC-CTCGAG-GTAAAGCGGATGCAACACTGT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACAGTGTTGCATCCGCTTTACCTCGAGGTAAAGCGGATGCAACACTGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACAGTGTTGCATCCGCTTTACCTCGAGGTAAAGCGGATGCAACACTGT-3'

Other clones with same target sequence:

(none)