Construct: shRNA TRCN0000425606

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030687.1-348s21c1
DNA Barcode:
AGGCATTTCTGTTGGCATTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slco1a4 (28250)
Gene Description:
solute carrier organic anion transporter family, member 1a4
Transcript:
RefSeq NM_030687.1 (CURRENT)
Match location:
Position 348 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000425606)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGGCATTTCTGTTGGCATTAA
Hairpin Sequence:
5'-CCGG-AGGCATTTCTGTTGGCATTAA-CTCGAG-TTAATGCCAACAGAAATGCCT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGGCATTTCTGTTGGCATTAACTCGAGTTAATGCCAACAGAAATGCCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGGCATTTCTGTTGGCATTAACTCGAGTTAATGCCAACAGAAATGCCT-3'

Other clones with same target sequence:

(none)