Construct: shRNA TRCN0000425621

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177263.3-3589s21c1
DNA Barcode:
GAATCAAGTTGTTAGCATATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Zhx3 (320799)
Gene Description:
zinc fingers and homeoboxes 3
Transcript:
RefSeq NM_177263.3 (CURRENT)
Match location:
Position 3589 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000425621)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAATCAAGTTGTTAGCATATA
Hairpin Sequence:
5'-CCGG-GAATCAAGTTGTTAGCATATA-CTCGAG-TATATGCTAACAACTTGATTC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAATCAAGTTGTTAGCATATACTCGAGTATATGCTAACAACTTGATTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAATCAAGTTGTTAGCATATACTCGAGTATATGCTAACAACTTGATTC-3'

Other clones with same target sequence:

(none)