Construct: shRNA TRCN0000425709

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017791.2-2059s21c1
DNA Barcode:
GGGAATCAGTGGGACTATTTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FLVCR2 (55640)
Gene Description:
FLVCR heme transporter 2
Transcript:
RefSeq NM_017791.2 (NON-CURRENT)
Match location:
Position 2059 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000425709)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGGAATCAGTGGGACTATTTG
Hairpin Sequence:
5'-CCGG-GGGAATCAGTGGGACTATTTG-CTCGAG-CAAATAGTCCCACTGATTCCC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGGAATCAGTGGGACTATTTGCTCGAGCAAATAGTCCCACTGATTCCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGGAATCAGTGGGACTATTTGCTCGAGCAAATAGTCCCACTGATTCCC-3'

Other clones with same target sequence:

(none)