Construct: shRNA TRCN0000425724

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021822.2-1717s21c1
DNA Barcode:
ATTGTGCTCAATACACAGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
APOBEC3G (60489)
Gene Description:
apolipoprotein B mRNA editing enzyme catalytic subunit 3G
Transcript:
RefSeq NM_021822.2 (NON-CURRENT)
Match location:
Position 1717 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000425724)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATTGTGCTCAATACACAGAAA
Hairpin Sequence:
5'-CCGG-ATTGTGCTCAATACACAGAAA-CTCGAG-TTTCTGTGTATTGAGCACAAT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATTGTGCTCAATACACAGAAACTCGAGTTTCTGTGTATTGAGCACAATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATTGTGCTCAATACACAGAAACTCGAGTTTCTGTGTATTGAGCACAAT-3'

Other clones with same target sequence:

(none)