Construct: shRNA TRCN0000425850

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_012236.3-1896s21c1
DNA Barcode:
CAATGGACTCTGCCTCAAATC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SCMH1 (22955)
Gene Description:
Scm polycomb group protein homolog 1
Transcript:
RefSeq NM_012236.3 (NON-CURRENT)
Match location:
Position 1896 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000425850)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAATGGACTCTGCCTCAAATC
Hairpin Sequence:
5'-CCGG-CAATGGACTCTGCCTCAAATC-CTCGAG-GATTTGAGGCAGAGTCCATTG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAATGGACTCTGCCTCAAATCCTCGAGGATTTGAGGCAGAGTCCATTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAATGGACTCTGCCTCAAATCCTCGAGGATTTGAGGCAGAGTCCATTG-3'

Other clones with same target sequence:

(none)