Construct: shRNA TRCN0000425866

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_198489.1-547s21c1
DNA Barcode:
AGGTGGTTCGGTCTGTCTTAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CCDC84 (338657)
Gene Description:
coiled-coil domain containing 84
Transcript:
RefSeq NM_198489.1 (NON-CURRENT)
Match location:
Position 547 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000425866)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGGTGGTTCGGTCTGTCTTAG
Hairpin Sequence:
5'-CCGG-AGGTGGTTCGGTCTGTCTTAG-CTCGAG-CTAAGACAGACCGAACCACCT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGGTGGTTCGGTCTGTCTTAGCTCGAGCTAAGACAGACCGAACCACCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGGTGGTTCGGTCTGTCTTAGCTCGAGCTAAGACAGACCGAACCACCT-3'

Other clones with same target sequence:

(none)