Construct: shRNA TRCN0000425869

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009751.2-581s21c1
DNA Barcode:
CGACGAGTTCCGCAGCAAATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Bfsp1 (12075)
Gene Description:
beaded filament structural protein 1, in lens-CP94
Transcript:
RefSeq NM_009751.2 (CURRENT)
Match location:
Position 581 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000425869)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGACGAGTTCCGCAGCAAATA
Hairpin Sequence:
5'-CCGG-CGACGAGTTCCGCAGCAAATA-CTCGAG-TATTTGCTGCGGAACTCGTCG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGACGAGTTCCGCAGCAAATACTCGAGTATTTGCTGCGGAACTCGTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGACGAGTTCCGCAGCAAATACTCGAGTATTTGCTGCGGAACTCGTCG-3'

Other clones with same target sequence:

(none)