Construct: shRNA TRCN0000425915

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009072.2-826s21c1
DNA Barcode:
TACACTCCATGGGCTTAATAC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rock2 (19878)
Gene Description:
Rho-associated coiled-coil containing protein kinase 2
Transcript:
RefSeq NM_009072.2 (CURRENT)
Match location:
Position 826 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000425915)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TACACTCCATGGGCTTAATAC
Hairpin Sequence:
5'-CCGG-TACACTCCATGGGCTTAATAC-CTCGAG-GTATTAAGCCCATGGAGTGTA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTACACTCCATGGGCTTAATACCTCGAGGTATTAAGCCCATGGAGTGTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATACACTCCATGGGCTTAATACCTCGAGGTATTAAGCCCATGGAGTGTA-3'

Other clones with same target sequence:

(none)