Construct: shRNA TRCN0000425927

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_138809.3-853s21c1
DNA Barcode:
TGGAACTGCTGTCCATCATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CMBL (134147)
Gene Description:
carboxymethylenebutenolidase homolog
Transcript:
RefSeq NM_138809.3 (NON-CURRENT)
Match location:
Position 853 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000425927)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGGAACTGCTGTCCATCATTT
Hairpin Sequence:
5'-CCGG-TGGAACTGCTGTCCATCATTT-CTCGAG-AAATGATGGACAGCAGTTCCA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGGAACTGCTGTCCATCATTTCTCGAGAAATGATGGACAGCAGTTCCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGGAACTGCTGTCCATCATTTCTCGAGAAATGATGGACAGCAGTTCCA-3'

Other clones with same target sequence:

(none)