Construct: shRNA TRCN0000425968

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011536.2-1602s21c1
DNA Barcode:
GTCATCCTTACAGTATCATTC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tbx4 (21387)
Gene Description:
T-box 4
Transcript:
RefSeq NM_011536.2 (CURRENT)
Match location:
Position 1602 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000425968)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCATCCTTACAGTATCATTC
Hairpin Sequence:
5'-CCGG-GTCATCCTTACAGTATCATTC-CTCGAG-GAATGATACTGTAAGGATGAC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCATCCTTACAGTATCATTCCTCGAGGAATGATACTGTAAGGATGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCATCCTTACAGTATCATTCCTCGAGGAATGATACTGTAAGGATGAC-3'

Other clones with same target sequence:

(none)