Construct: shRNA TRCN0000426098

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009684.2-701s21c1
DNA Barcode:
ATGGATCACATGATCAGTAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Apaf1 (11783)
Gene Description:
apoptotic peptidase activating factor 1
Transcript:
RefSeq NM_009684.2 (CURRENT)
Match location:
Position 701 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000426098)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATGGATCACATGATCAGTAAT
Hairpin Sequence:
5'-CCGG-ATGGATCACATGATCAGTAAT-CTCGAG-ATTACTGATCATGTGATCCAT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATGGATCACATGATCAGTAATCTCGAGATTACTGATCATGTGATCCATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATGGATCACATGATCAGTAATCTCGAGATTACTGATCATGTGATCCAT-3'

Other clones with same target sequence:

(none)