Construct: shRNA TRCN0000426137

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153608.1-1659s21c1
DNA Barcode:
ATTCATCTTGAGTAGACATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZNF114 (163071)
Gene Description:
zinc finger protein 114
Transcript:
RefSeq NM_153608.1 (NON-CURRENT)
Match location:
Position 1659 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000426137)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATTCATCTTGAGTAGACATTT
Hairpin Sequence:
5'-CCGG-ATTCATCTTGAGTAGACATTT-CTCGAG-AAATGTCTACTCAAGATGAAT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATTCATCTTGAGTAGACATTTCTCGAGAAATGTCTACTCAAGATGAATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATTCATCTTGAGTAGACATTTCTCGAGAAATGTCTACTCAAGATGAAT-3'

Other clones with same target sequence:

(none)