Construct: shRNA TRCN0000426145

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172817.3-1494s21c1
DNA Barcode:
CAGAGTGTGGCAAAGCCTTCA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Zfp647 (239546)
Gene Description:
zinc finger protein 647
Transcript:
RefSeq NM_172817.3 (CURRENT)
Match location:
Position 1494 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000426145)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGAGTGTGGCAAAGCCTTCA
Hairpin Sequence:
5'-CCGG-CAGAGTGTGGCAAAGCCTTCA-CTCGAG-TGAAGGCTTTGCCACACTCTG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGAGTGTGGCAAAGCCTTCACTCGAGTGAAGGCTTTGCCACACTCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGAGTGTGGCAAAGCCTTCACTCGAGTGAAGGCTTTGCCACACTCTG-3'

Other clones with same target sequence:

(none)