Construct: shRNA TRCN0000426208

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007556.2-1730s21c1
DNA Barcode:
AGAGCTTGTGGTTGCCATTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Bmp6 (12161)
Gene Description:
bone morphogenetic protein 6
Transcript:
RefSeq NM_007556.2 (NON-CURRENT)
Match location:
Position 1730 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000426208)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGAGCTTGTGGTTGCCATTAA
Hairpin Sequence:
5'-CCGG-AGAGCTTGTGGTTGCCATTAA-CTCGAG-TTAATGGCAACCACAAGCTCT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGAGCTTGTGGTTGCCATTAACTCGAGTTAATGGCAACCACAAGCTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGAGCTTGTGGTTGCCATTAACTCGAGTTAATGGCAACCACAAGCTCT-3'

Other clones with same target sequence:

(none)