Construct: shRNA TRCN0000426211

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003541.2-180s21c1
DNA Barcode:
GGTGTTCCTGGAGAACGTGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
H4C12 (8362)
Gene Description:
H4 clustered histone 12
Transcript:
RefSeq NM_003541.2 (CURRENT)
Match location:
Position 180 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000426211)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGTGTTCCTGGAGAACGTGAT
Hairpin Sequence:
5'-CCGG-GGTGTTCCTGGAGAACGTGAT-CTCGAG-ATCACGTTCTCCAGGAACACC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGTGTTCCTGGAGAACGTGATCTCGAGATCACGTTCTCCAGGAACACCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGTGTTCCTGGAGAACGTGATCTCGAGATCACGTTCTCCAGGAACACC-3'

Other clones with same target sequence:

(none)