Construct: shRNA TRCN0000426376

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_033626.2-1010s21c1
DNA Barcode:
CCACCTCCATCAGATCTTTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CCDC120 (90060)
Gene Description:
coiled-coil domain containing 120
Transcript:
RefSeq NM_033626.2 (NON-CURRENT)
Match location:
Position 1010 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000426376)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACCTCCATCAGATCTTTAT
Hairpin Sequence:
5'-CCGG-CCACCTCCATCAGATCTTTAT-CTCGAG-ATAAAGATCTGATGGAGGTGG-TTTTTTTGAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACCTCCATCAGATCTTTATCTCGAGATAAAGATCTGATGGAGGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACCTCCATCAGATCTTTATCTCGAGATAAAGATCTGATGGAGGTGG-3'

Other clones with same target sequence:

(none)