Construct: shRNA TRCN0000426399

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022906.2-941s21c1
DNA Barcode:
GAAAGGTGTGTAGTGACTATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
STAG3L4 (64940)
Gene Description:
stromal antigen 3-like 4 (pseudogene)
Transcript:
RefSeq NM_022906.2 (NON-CURRENT)
Match location:
Position 941 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000426399)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAAGGTGTGTAGTGACTATG
Hairpin Sequence:
5'-CCGG-GAAAGGTGTGTAGTGACTATG-CTCGAG-CATAGTCACTACACACCTTTC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAAGGTGTGTAGTGACTATGCTCGAGCATAGTCACTACACACCTTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAAGGTGTGTAGTGACTATGCTCGAGCATAGTCACTACACACCTTTC-3'

Other clones with same target sequence:

(none)