Construct: shRNA TRCN0000426405

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014759.3-1228s21c1
DNA Barcode:
ACCTCTACTTTGCGGACTTCT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PHYHIP (9796)
Gene Description:
phytanoyl-CoA 2-hydroxylase interacting protein
Transcript:
RefSeq NM_014759.3 (NON-CURRENT)
Match location:
Position 1228 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000426405)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACCTCTACTTTGCGGACTTCT
Hairpin Sequence:
5'-CCGG-ACCTCTACTTTGCGGACTTCT-CTCGAG-AGAAGTCCGCAAAGTAGAGGT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACCTCTACTTTGCGGACTTCTCTCGAGAGAAGTCCGCAAAGTAGAGGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACCTCTACTTTGCGGACTTCTCTCGAGAGAAGTCCGCAAAGTAGAGGT-3'

Other clones with same target sequence:

(none)