Construct: shRNA TRCN0000426428

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027712.3-3076s21c1
DNA Barcode:
TAATGTGTCTCAGGCATATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dlgap1 (224997)
Gene Description:
discs, large (Drosophila) homolog-associated protein 1
Transcript:
RefSeq NM_027712.3 (CURRENT)
Match location:
Position 3076 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000426428)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TAATGTGTCTCAGGCATATTT
Hairpin Sequence:
5'-CCGG-TAATGTGTCTCAGGCATATTT-CTCGAG-AAATATGCCTGAGACACATTA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTAATGTGTCTCAGGCATATTTCTCGAGAAATATGCCTGAGACACATTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATAATGTGTCTCAGGCATATTTCTCGAGAAATATGCCTGAGACACATTA-3'

Other clones with same target sequence:

(none)