Construct: shRNA TRCN0000426447

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018431.3-1245s21c1
DNA Barcode:
GCTTAAAGTCCTGGCTAATTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DOK5 (55816)
Gene Description:
docking protein 5
Transcript:
RefSeq NM_018431.3 (NON-CURRENT)
Match location:
Position 1245 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000426447)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTAAAGTCCTGGCTAATTG
Hairpin Sequence:
5'-CCGG-GCTTAAAGTCCTGGCTAATTG-CTCGAG-CAATTAGCCAGGACTTTAAGC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTAAAGTCCTGGCTAATTGCTCGAGCAATTAGCCAGGACTTTAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTAAAGTCCTGGCTAATTGCTCGAGCAATTAGCCAGGACTTTAAGC-3'

Other clones with same target sequence:

(none)