Construct: shRNA TRCN0000426464

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172003.3-1657s21c1
DNA Barcode:
CAACTTTCAACATTGTCATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CBWD2 (150472)
Gene Description:
COBW domain containing 2
Transcript:
RefSeq NM_172003.3 (CURRENT)
Match location:
Position 1657 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000426464)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAACTTTCAACATTGTCATTT
Hairpin Sequence:
5'-CCGG-CAACTTTCAACATTGTCATTT-CTCGAG-AAATGACAATGTTGAAAGTTG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAACTTTCAACATTGTCATTTCTCGAGAAATGACAATGTTGAAAGTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAACTTTCAACATTGTCATTTCTCGAGAAATGACAATGTTGAAAGTTG-3'

Other clones with same target sequence:

(none)