Construct: shRNA TRCN0000426492

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019517.4-1832s21c1
DNA Barcode:
GCATTGGGCTTCAGACTTTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Bace2 (56175)
Gene Description:
beta-site APP-cleaving enzyme 2
Transcript:
RefSeq NM_019517.4 (NON-CURRENT)
Match location:
Position 1832 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000426492)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATTGGGCTTCAGACTTTAT
Hairpin Sequence:
5'-CCGG-GCATTGGGCTTCAGACTTTAT-CTCGAG-ATAAAGTCTGAAGCCCAATGC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATTGGGCTTCAGACTTTATCTCGAGATAAAGTCTGAAGCCCAATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATTGGGCTTCAGACTTTATCTCGAGATAAAGTCTGAAGCCCAATGC-3'

Other clones with same target sequence:

(none)