Construct: shRNA TRCN0000426535

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_052870.2-2290s21c1
DNA Barcode:
GTAGAGGAATTAAGATCAATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SNX18 (112574)
Gene Description:
sorting nexin 18
Transcript:
RefSeq NM_052870.2 (NON-CURRENT)
Match location:
Position 2290 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000426535)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTAGAGGAATTAAGATCAATT
Hairpin Sequence:
5'-CCGG-GTAGAGGAATTAAGATCAATT-CTCGAG-AATTGATCTTAATTCCTCTAC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTAGAGGAATTAAGATCAATTCTCGAGAATTGATCTTAATTCCTCTACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTAGAGGAATTAAGATCAATTCTCGAGAATTGATCTTAATTCCTCTAC-3'

Other clones with same target sequence:

(none)