Construct: shRNA TRCN0000426586

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003604.2-2691s21c1
DNA Barcode:
TAAGAGACCTAACCGACTTTC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
IRS4 (8471)
Gene Description:
insulin receptor substrate 4
Transcript:
RefSeq NM_003604.2 (CURRENT)
Match location:
Position 2691 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000426586)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TAAGAGACCTAACCGACTTTC
Hairpin Sequence:
5'-CCGG-TAAGAGACCTAACCGACTTTC-CTCGAG-GAAAGTCGGTTAGGTCTCTTA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTAAGAGACCTAACCGACTTTCCTCGAGGAAAGTCGGTTAGGTCTCTTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATAAGAGACCTAACCGACTTTCCTCGAGGAAAGTCGGTTAGGTCTCTTA-3'

Other clones with same target sequence:

(none)