Construct: shRNA TRCN0000426588

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003518.3-199s21c1
DNA Barcode:
CGTTAACGACATCTTCGAACG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
H2BC8 (8339)
Gene Description:
H2B clustered histone 8
Transcript:
RefSeq NM_003518.3 (CURRENT)
Match location:
Position 199 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000426588)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTTAACGACATCTTCGAACG
Hairpin Sequence:
5'-CCGG-CGTTAACGACATCTTCGAACG-CTCGAG-CGTTCGAAGATGTCGTTAACG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTTAACGACATCTTCGAACGCTCGAGCGTTCGAAGATGTCGTTAACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTTAACGACATCTTCGAACGCTCGAGCGTTCGAAGATGTCGTTAACG-3'

Other clones with same target sequence:

(none)