Construct: shRNA TRCN0000426636

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_194278.3-3388s21c1
DNA Barcode:
GAGTTCTACTACACCTACAAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ELMSAN1 (91748)
Gene Description:
ELM2 and Myb/SANT domain containing 1
Transcript:
RefSeq NM_194278.3 (CURRENT)
Match location:
Position 3388 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000426636)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGTTCTACTACACCTACAAG
Hairpin Sequence:
5'-CCGG-GAGTTCTACTACACCTACAAG-CTCGAG-CTTGTAGGTGTAGTAGAACTC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGTTCTACTACACCTACAAGCTCGAGCTTGTAGGTGTAGTAGAACTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGTTCTACTACACCTACAAGCTCGAGCTTGTAGGTGTAGTAGAACTC-3'

Other clones with same target sequence:

(none)