Construct: shRNA TRCN0000426779
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_006909.1-3913s21c1
- DNA Barcode:
- GAAGGTGAAAGATGGCTATTT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- RASGRF2 (5924)
- Gene Description:
- Ras protein specific guanine nucleotide releasing factor 2
- Transcript:
- RefSeq NM_006909.1 (NON-CURRENT)
- Match location:
- Position 3913 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000426779)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GAAGGTGAAAGATGGCTATTT
- Hairpin Sequence:
-
5'-CCGG-GAAGGTGAAAGATGGCTATTT-CTCGAG-AAATAGCCATCTTTCACCTTC-TTTTTGGAAT-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGAAGGTGAAAGATGGCTATTTCTCGAGAAATAGCCATCTTTCACCTTCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGAAGGTGAAAGATGGCTATTTCTCGAGAAATAGCCATCTTTCACCTTC-3'
Other clones with same target sequence:
(none)
