Construct: shRNA TRCN0000426824

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003913.4-2603s21c1
DNA Barcode:
GCTTCACATGTTGCGGATAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PRPF4B (8899)
Gene Description:
pre-mRNA processing factor 4B
Transcript:
RefSeq NM_003913.4 (NON-CURRENT)
Match location:
Position 2603 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000426824)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTCACATGTTGCGGATAAT
Hairpin Sequence:
5'-CCGG-GCTTCACATGTTGCGGATAAT-CTCGAG-ATTATCCGCAACATGTGAAGC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTCACATGTTGCGGATAATCTCGAGATTATCCGCAACATGTGAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTCACATGTTGCGGATAATCTCGAGATTATCCGCAACATGTGAAGC-3'

Other clones with same target sequence:

(none)