Construct: shRNA TRCN0000426869

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000052.4-4781s21c1
DNA Barcode:
TTCTATTTGAGTTGCGTTTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ATP7A (538)
Gene Description:
ATPase copper transporting alpha
Transcript:
RefSeq NM_000052.4 (NON-CURRENT)
Match location:
Position 4781 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000426869)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTCTATTTGAGTTGCGTTTAT
Hairpin Sequence:
5'-CCGG-TTCTATTTGAGTTGCGTTTAT-CTCGAG-ATAAACGCAACTCAAATAGAA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTCTATTTGAGTTGCGTTTATCTCGAGATAAACGCAACTCAAATAGAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTCTATTTGAGTTGCGTTTATCTCGAGATAAACGCAACTCAAATAGAA-3'

Other clones with same target sequence:

(none)