Construct: shRNA TRCN0000426962

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014943.3-1494s21c1
DNA Barcode:
GTTTGCCACCCAGCGCTTAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZHX2 (22882)
Gene Description:
zinc fingers and homeoboxes 2
Transcript:
RefSeq NM_014943.3 (NON-CURRENT)
Match location:
Position 1494 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000426962)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTTTGCCACCCAGCGCTTAAA
Hairpin Sequence:
5'-CCGG-GTTTGCCACCCAGCGCTTAAA-CTCGAG-TTTAAGCGCTGGGTGGCAAAC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTTTGCCACCCAGCGCTTAAACTCGAGTTTAAGCGCTGGGTGGCAAACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTTTGCCACCCAGCGCTTAAACTCGAGTTTAAGCGCTGGGTGGCAAAC-3'

Other clones with same target sequence:

(none)