Construct: shRNA TRCN0000427002

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175363.3-739s21c1
DNA Barcode:
GGATCCACGGCACCCTTATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pphln1 (223828)
Gene Description:
periphilin 1
Transcript:
RefSeq NM_175363.3 (NON-CURRENT)
Match location:
Position 739 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000427002)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGATCCACGGCACCCTTATTT
Hairpin Sequence:
5'-CCGG-GGATCCACGGCACCCTTATTT-CTCGAG-AAATAAGGGTGCCGTGGATCC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGATCCACGGCACCCTTATTTCTCGAGAAATAAGGGTGCCGTGGATCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGATCCACGGCACCCTTATTTCTCGAGAAATAAGGGTGCCGTGGATCC-3'

Other clones with same target sequence:

(none)