Construct: shRNA TRCN0000427036

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020895.3-2458s21c1
DNA Barcode:
ACGGAACCGAGATGCACTTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GRAMD1A (57655)
Gene Description:
GRAM domain containing 1A
Transcript:
RefSeq NM_020895.3 (NON-CURRENT)
Match location:
Position 2458 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000427036)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACGGAACCGAGATGCACTTTA
Hairpin Sequence:
5'-CCGG-ACGGAACCGAGATGCACTTTA-CTCGAG-TAAAGTGCATCTCGGTTCCGT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACGGAACCGAGATGCACTTTACTCGAGTAAAGTGCATCTCGGTTCCGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACGGAACCGAGATGCACTTTACTCGAGTAAAGTGCATCTCGGTTCCGT-3'

Other clones with same target sequence:

(none)